The game has been arranged as part of the Football Associations 125th anniversary celebrations and will be the first time the two sides have met at Under-21 level since 1993.
Blades boss David Weir said “England versus Scotland at any level is a prestigious event and it is great news that our historic home has been selected to host a fixture between the Auld Enemy.”
The game between the two sides will take place on Tuesday, 13th August 2013.